Effects of 1,25(OH) 2 D 3 and 25(OH)D 3 on C2C12 Myoblast Proliferation, Differentiation, and Myotube Hypertrophy
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Lactoferrin promotes murine C2C12 myoblast proliferation and differentiation and myotube hypertrophy
Lactoferrin (Lf) is a multifunctional glycoprotein, which promotes the proliferation of murine C2C12 myoblasts. In the present study, it was investigated how Lf promotes myoblast proliferation and whether Lf promotes myoblast differentiation or induces myotube hypertrophy. Lf promoted the proliferation of myoblasts in a dose‑dependent manner. Myoblast proliferation increased on day 3 when myobl...
متن کاملEffects of 1,25(OH)2D3 and 25(OH)D3 on C2C12 Myoblast Proliferation, Differentiation, and Myotube Hypertrophy
An adequate vitamin D status is essential to optimize muscle strength. However, whether vitamin D directly reduces muscle fiber atrophy or stimulates muscle fiber hypertrophy remains subject of debate. A mechanism that may affect the role of vitamin D in the regulation of muscle fiber size is the local conversion of 25(OH)D to 1,25(OH)2 D by 1α-hydroxylase. Therefore, we investigated in a murin...
متن کاملEnhancement of C2C12 myoblast proliferation and differentiation by GASP-2, a myostatin inhibitor
BACKGROUND GASP-2 is a secreted multi-domain glycoprotein known as a specific inhibitor of myostatin and GDF-11. Here we investigate the role of GASP-2 on myogenesis and the effect of its glycosylation on its activity. METHODS GASP-2 overexpression or knockdown by shRNAs were carried out on C2C12 myoblasts cells. In silico analysis of GASP-2 protein was performed to identify its glycosylation...
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Exosomes are nanometer-sized microvesicles formed in multivesicular bodies (MVBs) during endosome maturation. Exosomes are released from cells into the microenvironment following fusion of MVBs with the plasma membrane. During the last decade, skeletal muscle-secreted proteins have been identified with important roles in intercellular communications. To investigate whether muscle-derived exosom...
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Vyas, Dharmesh R., Espen E. Spangenburg, Tsghe W. Abraha, Thomas E. Childs, and Frank W. Booth. GSK-3 negatively regulates skeletal myotube hypertrophy. Am J Physiol Cell Physiol 283: C545–C551, 2002. First published April 3, 2002; 10.1152/ajpcell.00049.2002.—To determine whether changes in glycogen synthase kinase-3 (GSK-3 ) phosphorylation contribute to muscle hypertrophy, we delineated the e...
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ژورنال
عنوان ژورنال: Journal of Cellular Physiology
سال: 2016
ISSN: 0021-9541,1097-4652
DOI: 10.1002/jcp.25388